A tunable system in which transcriptional regulation by PU.1 can be investigated using PU.1-deficient myeloid progenitors and a conditionally activatable PU.1-estrogen receptor binding domain fusion protein PUER developed by Walsh et al. These myeloid progenitors undergo rapid cell cycle arrest and differentiate into macrophages upon addition of 4-hydroxy-tamoxifen (OHT).
J.C. Walsh, R.P. DeKoter, H.J. Lee, E.D. Smith, D.W. Lancki, M.F. Gurish, D.S. Friend, R.L. Stevens, J. Anastasi, H. Singh, Cooperative and antagonistic interplay between PU.1 and GATA-2 in the specification of myeloid cell fates, Immunity 17 (2002) 665â676.
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