FastQCFastQC Report
Sat 27 Sep 2014
SRR351413.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SRR351413.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 31295488
Filtered Sequences 0
Sequence length 36
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 453293 1.448429243218703 No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 60334 0.19278817444866173 No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 51573 0.1647937236192003 No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 37763 0.12066595670276814 No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 34167 0.1091754824209803 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 2045930 3.8377244 4.6425085 25
AAAAA 2222770 3.738055 4.7598042 2
CCCAG 4193940 3.2508895 3.8258884 24
GGCTG 3687175 3.0413337 3.568424 4
CTGCT 1993570 1.9690876 5.291269 27
TTGAA 1219290 1.7918519 6.1105742 31
GGAAG 1819245 1.7908367 8.747537 5
TCTGC 1803135 1.7809911 5.125433 26
CTTGA 1444280 1.7400659 5.24049 30
TGAAA 1203735 1.730771 5.8352933 32
TTCTG 1397840 1.7213062 5.98773 25
TGCTT 1333810 1.6424593 5.9131618 28
CTTCT 1342185 1.6197988 5.8181443 24
AGCTC 1510965 1.4601638 5.0103226 10
GAAGA 1221660 1.4376196 9.272091 6
AAGAG 1218855 1.4343185 8.183863 7
GAGCT 1404945 1.3853467 5.414063 9
TCTTC 1115365 1.346064 5.437703 23
GTCTT 1062450 1.3083054 5.344938 22
AGAGC 1292865 1.2472857 5.3529463 8
GATCG 638770 0.62985945 7.5156384 1
CGGAA 567270 0.5472712 7.387327 4
TCGGA 484025 0.47727308 7.49744 3
ATCGG 460980 0.45454955 7.4886956 2