FastQCFastQC Report
Sat 27 Sep 2014
SRR343292.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SRR343292.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29057790
Filtered Sequences 0
Sequence length 36
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 99101 0.3410479599446482 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCCCC 2933885 5.203897 7.6470594 32
AAAAA 4639555 3.0163217 3.3728712 2
GGAAG 1573330 2.2133207 14.604451 5
CTGCT 1590645 1.9934208 6.156961 27
TTCTG 1886440 1.9317756 5.46812 25
TCTGC 1475905 1.8496269 6.0357428 26
GAAGA 1609830 1.7509085 10.665851 6
CTTCT 1760575 1.7037426 5.087091 24
TGCTT 1598925 1.6373509 5.076927 28
AAGAG 1442650 1.5690776 8.971801 7
ATGCC 1190965 1.4943762 6.0466776 17
AGAGC 1030620 1.3701208 7.486598 8
GAGCT 976620 1.2967335 7.064177 9
AGCTC 993470 1.2465671 5.9733133 10
TGCCG 687360 1.1155429 6.8951936 18
GATCG 677130 0.89907765 12.677551 1
GCCGT 552545 0.89674646 6.4574237 19
CGGAA 673825 0.89579254 12.609722 4
CCGTC 575910 0.8832679 6.1397476 20
ATCGG 617765 0.82025415 12.623504 2
GCTCG 475355 0.77147186 6.6885595 11
TCGGA 562120 0.74637 12.421585 3
CTCGT 445900 0.55880874 5.0244517 12