FastQCFastQC Report
Sat 27 Sep 2014
SRR351410.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SRR351410.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24988334
Filtered Sequences 0
Sequence length 36
%GC 50

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 82395 0.3297338670117023 No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 36122 0.1445554553576881 No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 30799 0.12325351502024905 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 3258705 4.4849033 6.1603084 3
GTGTG 3084970 4.1755996 5.7244854 2
ACACA 3114465 3.833467 4.0752363 6
CACAC 2997960 3.7133312 4.0097404 1