FastQCFastQC Report
Sat 27 Sep 2014
SRR351409.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename SRR351409.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 20495912
Filtered Sequences 0
Sequence length 36
%GC 50

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 44150 0.21540880932744053 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 2265335 3.7378716 4.4149237 3
ACACA 2340880 3.5963857 4.072322 2
GTGTG 2097790 3.4060812 3.9917064 30
CACAC 2229395 3.367441 4.118754 1