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Promoting Discovery and Reproducibility in Stem Cell Research

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    Mus musculus
    Kelly McNagny | 12/21/2011 - 16:16
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    We are interested in comparing expression patterns of hematopoletic stem cells, mast cell precursors and mature mast cells. Our group recently reported that murine mast cells express CD34, Sca-1 and c-kit. Microarray analysis may uncover other novel surface antigens useful in separating mast cells from stem cells.
    Mus musculus
    John Hassell | 12/21/2011 - 17:49
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    Mammary gland cells were isolated from 5-8 week old FVB mice and allowed to proliferate as undifferentiated mammospheres in the presence of growth factors (bFGF and EGF). Comparison of the gene expression profiles of undifferentiated mammospheres vs. mammospheres that were allowed to differentiate for 6 days by the removal of growth factors will help to identify genes that are implicated in the maintenance of the mammary gland stem cell compartment.
    Mus musculus
    Michael Rudnicki | 12/21/2011 - 18:03
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    An 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days.
    Mus musculus
    Derrick Rancourt | 12/21/2011 - 18:46
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    Differentiation Time Course; examination of osteoblast differentiation by comparing cells exposed to growth factors with control cells.
    Homo sapiens
    Mickie Bhatia | 12/31/2011 - 16:46
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133A Array
    Smad7 is a member of the TGF-B superfamily that plays a signigicant role in fate determination of hematopoietic stem cells. This experiment compares expression between megakaryotic and fibroblast cell lines that have been transduced with either Smad7 recombinant retroviral vector or retroviral vector alone.
    Mus musculus
    Jacques Galipeau | 12/31/2011 - 17:58
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    The role of bone marrow stromal cells in angiogenesis and tissue regeneration has previously been assessed by the use of heterogenous populations. This experiment is designed to assess the angiogenic potential of homogenous, well characterized clones and to provide gene expression profiling for these clones.
    Homo sapiens
    Rod McInnes | 12/31/2011 - 18:17
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133A Array
    The pigmented portion of ciliary epithelium in the adult mammalian eye harbors mitotically quiescent retinal sphere cells, which are capable of self-renewal and differentiating into retinal neurons when assayed in vitro; however, very little is known about the molecular mechanism controlling the proliferation and differentiation of these adult retinal stem cells or their molecular resemblance to mutipotent stem/progenitor cells during early eye development. This experiment studies the gene...
    Mus musculus
    Ruth Slack | 12/31/2011 - 18:50
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    The ganglionic eminence was dissected and neurospheres were cultured from the brain of E13.5 embroys. RNA was isolated from neurospheres cultured from p107-/- embryos and their wildtype littermates. P107 knockout mice have previously been shown to have an increased number of stem cells and enhanced stem cell self renewal. With the microarray experiment we are hoping to discover the genes involved in stem cell number and self renewal along with p107.
    Mus musculus
    Michael Rudnicki | 12/31/2011 - 19:53
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    Gene expression profiles of sorted bone marrow samples to determine genes implicated in hematopoetic stem cell identity.
    Homo sapiens
    Peter Zandstra | 12/31/2011 - 19:59
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133A Array
    Human ES cell line I-6 was cultured in standard conditions to maintain an undifferentiated, self renewing phenotype. The cells were sorted based upon their expression of SSEA4, which is a marker of the undifferentiated, self renewing phenotype.

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