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    Mus musculus
    Pu Zhang | 12/17/2010 - 10:46
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Knockdown of the transcription factor PU.1 (Spi1) leads to acute myeloid leukemia (AML) in mice. We examined the transcriptome of PU.1 knockdown hematopoietic stem cells (HSC) in the preleukemic phase by linear amplification and genome-wide array analysis to identify transcriptional changes preceding malignant transformation. Hierarchical cluster analysis and principal component analysis clearly distinguished PU.1 knockdown from wildtype HSC. Jun family transcription factors c-Jun and JunB were...
    Mus musculus
    Irving Weissman | 12/17/2010 - 10:47
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the...
    Mus musculus
    Babacar Cisse | 12/17/2010 - 10:47
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Analysis of expression profiles of pDCs from wild type and heterozygous E2-2 mice. Results show the control by E2-2 of the expression of pDC-enriched genes. Duplicates of both wild type and heterozygous pDCs were used.
    Mus musculus
    Teresita Arenzana | 12/17/2010 - 10:56
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    The development, homeostasis and function of B lymphocytes involve multiple rounds of B cell receptor (BCR)-controlled proliferation and prolonged maintenance. We analyzed the role of transcription factor Zfx, a recently identified regulator of stem cell maintenance, in B cell development and homeostasis. Conditional Zfx deletion in the bone marrow blocked B cell development at the pre-BCR selection checkpoint. Zfx deficiency in peripheral B cells caused impaired generation of the B-1 cell...
    Homo sapiens
    Roel Verhaak | 04/01/2011 - 01:30
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133 Plus 2.0 Array
    The pretreatment karyotype of leukemic blasts is currently the key determinant in therapy decision-making in acute myeloid leukemia (AML). However, approximately fifty percent of AML patients, often carrying a normal karyotype, are currently unclassifiable based these established methods. Gene expression profiling has proven to be valuable for risk stratification of AML. The gene expression profiles of AML samples of two independent cohorts (n=247 and n=214) were determined using Affymetrix...
    Mus musculus
    Scott Armstrong | 04/05/2011 - 12:19
    Histone Modification (ChIP-Seq)
    Illumina Genome Analyzer IIx
    Profiling of histone modification, transcription factor binding of MLL-AF9 of HSCs, GMPs and L-GMPs. The histone 3 lysine 79 (H3K79) methyltransferase Dot1l has been implicated in the development of leukemias bearing translocations that involve the Mixed Lineage Leukemia (MLL) gene. We identified the MLL-fusion targets in a murine MLL-AF9 leukemia model, and conducted epigenetic profiling for H3K79me2, H3K4me3, H3K27me3 and H3K36me3. Histone methylation patterns are highly abnormal on MLL-AF9...
    Homo sapiens
    External Researcher | 10/20/2011 - 11:21
    Transcription Profiling (RNA-Seq)
    Illumina Genome Analyzer
    Alternative splicing (AS) is a key process underlying the expansion of proteomic diversity and the regulation of gene expression. However, the contribution of AS to the control of embryonic stem cell (ESC) pluripotency is not well understood. Here, we identify an evolutionarily conserved ESC-specific AS event that changes the DNA binding preference of the forkhead family transcription factor FOXP1. We show that the ESC-specific isoform of FOXP1 stimulates the expression of transcription factor...
    Mus musculus
    Scott Armstrong | 12/13/2011 - 09:40
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430A 2.0 Array
    Leukemia stem cells (LSCs) are capable of limitless self-renewal and are responsible for the maintenance of leukemia. Because selective eradication of LSCs could offer substantial therapeutic benefit, there is interest in identifying the signaling pathways that control their development. We studied LSCs in mouse models of acute myelogenous leukemia (AML) induced either by coexpression of the Hoxa9 and Meis1a oncogenes or by the fusion oncoprotein MLL-AF9. We show that the Wnt/beta-catenin...
    Mus musculus
    Andrei Krivtsov | 12/13/2011 - 11:25
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430A 2.0 Array
    Mll-AF4stop knock-in mice were treated with 5-FU and 5 days later their bone marrow infected ex vivo with either Cre-GFP to activate the Mll-AF4 fusion construct or with a control MIG-Cre retrovirus. GFP+ cells were sorted 2 days post-infection and cultured for 14 days under lymphoid growth conditions before total RNA was isolated for hybridization to Affymetrix expression microarrays.
    Mus musculus
    Ronald DePinho | 12/15/2011 - 14:20
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Glioblastoma (GBM) is a highly lethal brain tumour presenting as one of two subtypes with distinct clinical histories and molecular profiles. The primary GBM subtype presents acutely as a high-grade disease that typically harbours mutations in EGFR, PTEN and INK4A/ARF (also known as CDKN2A), and the secondary GBM subtype evolves from the slow progression of a low-grade disease that classically possesses PDGF and TP53 events. Here we show that concomitant central nervous system (CNS)-specific...

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