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    Mus musculus
    Scott Armstrong | 12/17/2010 - 10:36
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Study the effect of the deficiency of FoxO1, FoxO3, and FoxO4 in murine bone marrow hematopoietic stem cells and myeloid progenitors on expression of genes involved in reactive oxygen species (ROS) metabolism.
    Mus musculus
    Jennifer Trowbridge | 12/17/2010 - 10:37
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430A 2.0 Array
    To examine the role of Dnmt1 in adult hematopoietic stem cells (HSCs), we conditionally disrupted Dnmt1 in the hematopoietic system. Dnmt1 was conditionally deleted by injections of poly(I)-poly(C) to induce Cre expression from the Mx-Cre transgene. Control mice were also injected with poly(I)-poly(C) but do not carry the Mx-Cre transgene. Four days after completion of poly(I)-poly(C) injections, bone marrow was harvested from the mice, antibody-mediated magnetic bead selection was used to...
    Mus musculus
    Nathan Boles | 12/17/2010 - 10:40
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Hematopoietic stem cells (HSC) continuously regenerate a complete hematologic and immune system. Very few genes that regulate this process have yet been identified. In order to identify factors governing differentiation, we have compared the transcriptome of highly purified HSC with their differentiated progeny, including erythrocytes, granulocytes, monocytes, NK cells, activated and naïve T-cells, and B-cells. Chromosomal analysis revealed that HSC were more transcriptionally active than other...
    Mus musculus
    Jochen Hartner | 12/17/2010 - 10:40
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Hematopoietic stem cells purified from +/+, +/- and -/- ADAR1 mice were compared with global gene expression analysis. Each sample was purified from one mouse.
    Mus musculus
    Andreas Trumpp | 12/17/2010 - 10:40
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Bone marrow hematopoietic stem cells (HSCs) are crucial to maintain lifelong production of all blood cells. Although HSCs divide infrequently, it is thought that the entire HSC pool turns over every few weeks, suggesting that HSCs regularly enter and exit cell cycle. Here, we combine flow cytometry with label-retaining assays (BrdU and histone H2B-GFP) to identify a population of dormant mouse HSCs (d-HSCs) within the lin(-)Sca1+cKit+CD150+CD48(-)CD34(-) population. Computational modeling...
    Mus musculus
    Tim Somervaille | 12/17/2010 - 10:40
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Normal myeloid lineage cell populations (C57BL/6 mice, aged 4-10 weeks, male or female) with three distinct immunophenotypes were prospectively isolated and characterized. In preparation for FACS sorting, bone marrow cells were separated into c-kit+ and c-kit- fractions using an AutoMACS device. C-kit+ cells were further fractionated based on Gr1 and Mac1 expression, and absence of lineage antigen expression (B220, TER119, CD3, CD4, CD8 and IL7Rα), by cell sorting. C-kit+ Gr1+ Mac1lo/- and c-...
    Mus musculus
    Tri Giang Phan | 12/17/2010 - 10:41
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Lymph node (LN) resident macrophages lining the lymphatic sinuses play critical roles in antigen capture and presentation as well as degradation. We used microarray to examine global gene expression profiles to compare subcapsular sinus (SCS) and medullary (med) macrophages to determine the underlying molecular basis of their differential handling of antigens. Primary SCS and med macrophages were isolated and purified from LNs by flourescence-activated cell sorting and RNA extracted,...
    Mus musculus
    Andreas Trumpp | 12/17/2010 - 10:44
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Maintenance of the blood system is dependent on dormant haematopoietic stem cells (HSCs) with long-term self-renewal capacity. Upon injury these cells are induced to proliferate in order to quickly re-establish homeostasis. The signalling molecules promoting the exit of HSCs out of the dormant stage remain largely unknown. Here we show that in response to treatment of mice with interferon-alpha (IFNα), HSCs efficiently exit G0 and enter an active cell cycle. HSCs respond to IFNα treatment by...
    Mus musculus
    Jiangwen Zhang | 12/17/2010 - 10:44
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Regulation of lineage potential and transcriptional priming by Ikaros. New insight is provided into a bivalent regulation of lineage priming in the HSC and its lympho-myeloid restricted progeny the LMPP by the lymphoid lineage-determining factor Ikaros. Whereas Ikaros is responsible for the activation of a cascade of lymphoid expression programs and for the establishment of lymphoid potential from the HSC to the LMPP it is also responsible for the repression of stem cell and erythroid genetic...
    Mus musculus
    Thomas Malek | 12/17/2010 - 10:44
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Interleukin-2 receptor (IL-2R) signaling is essential for T regulatory (Treg) cell development and homeostasis. Here we show that expression of IL-2Rbeta chains that lack tyrosine residues important for the association of the adaptor Shc and the transcription factor STAT5 in IL-2Rbeta-deficient mice resulted in production of a normal proportion of natural Treg cells that suppressed severe autoimmunity related with deficiency in IL-2 or IL-2R. These mutant IL-2Rbeta chains supported suboptimal...

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