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    Mus musculus
    Yi Zhang | 02/05/2015 - 11:46
    Transcription Factor Binding (ChIP-Seq)
    Illumina Genome Analyzer
    Polycomb group (PcG) proteins control organism development by regulating the expression of developmental genes. Transcriptional regulation by PcG proteins is achieved at least partly through the PRC2-mediated methylation on lysine 27 of histone H3 (H3K27) and PRC1-mediated ubiquitylation on lysine 119 of histone H2A (uH2A). As an integral component of PRC1, Bmi1 has been demonstrated to be critical for H2A ubiquitylation. Here we report genome wide localization of the Bmi1-dependent uH2A mark...
    Mus musculus
    William Pu | 02/05/2015 - 15:27
    Transcription Factor Binding (ChIP-Seq)
    Illumina Genome Analyzer
    We performed bio-ChIP-seq using streptavidin beads immunoprecipitation of biotinylated 5 cardiac transcription factors (fbio) and P300 antibody ChIP-seq. We used a dox-inducible dual adenovirus system to express biotinylated Core Cardiac TFs in HL1. One adenovirus expressed the dox-activated reverse tet activator protein (rtTA) and the E. coli biotinylating enzyme BirA from the cardiac specific rat troponin T promoter. The second virus expressed a Core Cardiac TF fused to a C-terminal flag-bio...
    Mus musculus
    Ramesh Shivdasani | 02/05/2015 - 16:16
    Transcription Factor Binding (ChIP-Seq)
    Illumina Genome Analyzer II
    We conditionally inactivated mouse Cdx2, a dominant regulator of intestinal development, and mapped its genome occupancy in adult intestinal villi. Although homeotic transformation, observed in Cdx2-null embryos, was absent in mutant adults, gene expression and cell morphology were vitally compromised. Lethality was accelerated in mice lacking both Cdx2 and its homolog Cdx1, with exaggeration of defects in crypt cell replication and enterocyte differentiation. Cdx2 occupancy correlated with...
    Mus musculus
    Amit Sinha | 02/11/2015 - 10:31
    Transcription Factor Binding (ChIP-Seq)
    Illumina HiSeq 2000
    Leukemic cells from primary and secondary recipients of MLL-AF9-transduced WT and Ezh2-null cells were sorted for GFP and YFP expression and analyzed by Chip-Seq.
    Homo sapiens
    Richard A. Young | 02/25/2015 - 16:53
    Transcription Factor Binding (ChIP-Seq)
    Illumina Genome Analyzer II
    Human CD133-positive Umbilical Cord Blood (UCB) cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), PU.1/SPI1 (Santa Cruz SC-352X), IKAROS (Santa Cruz SC-9859X) and MEIS1 (Abcam ab19867).
    Mus musculus
    Derrick Rossi | 12/17/2010 - 10:32
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Loss of immune function and an increased incidence of myeloid leukemia are two of the most clinically significant consequences of aging of the hematopoietic system. To better understand the mechanisms underlying hematopoietic aging, we evaluated the cell intrinsic functional and molecular properties of highly purified long-term hematopoietic stem cells (LT-HSCs) from young and old mice. We found that LT-HSC aging was accompanied by cell autonomous changes, including increased stem cell self-...
    Mus musculus
    Andrei Krivtsov | 12/17/2010 - 10:32
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430A 2.0 Array
    Leukaemias and other cancers possess a rare population of cells capable of the limitless self-renewal necessary for cancer initiation and maintenance. Here we show that leukaemia stem cells (LSC) can maintain the global identity of the progenitor from which they arose while activating a limited stem-cell- or self-renewal-associated programme. We isolated LSC from leukaemias initiated in committed granulocyte macrophage progenitors through introduction of the MLL-AF9 fusion protein encoded by...
    Mus musculus
    Elisa Laurenti | 12/17/2010 - 10:33
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Analysis of HSCs from control and c-myc N-myc deficient long-term hematopoietic stem cells. HSCs lacking both c-myc and N-myc display increased apoptosis rates. Data provide insight into the molecular changes occuring upon complete loss of Myc activity, clarifying the resulting apoptotic mechanism and the role of Myc family proteins in HSCs. LT-HSC (Lin-Sca1+CD150+CD48-) cells were sorted from the BM of MxCre c-myc flox2 N-myc flox2 (experimental) and c-myc flox2 N-myc flox2 (control) mice...
    Mus musculus
    Robert Mansson | 12/17/2010 - 10:36
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Lineage negative Kit+ Sca1+ (LSK) hematopoietic stem cell subpopulations were FACS sorted based on their CD34/FLT3 expression. Subsequently RNA was extracted, labeled and hybridized to Affymetrix microarrays. We sought to obtain expression pattern changes that might explain the difference in function between the functionally distinct LSK subpopulations.
    Mus musculus
    Francesca Ficara | 12/17/2010 - 10:36
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    Self-renewal is a defining characteristic of stem cells, however the molecular pathways underlying its regulation are poorly understood. Here we demonstrate that conditional inactivation of the Pbx1 proto-oncogene in the hematopoietic compartment results in a progressive loss of long-term hematopoietic stem cells (LT-HSCs) that is associated with concomitant reduction in their quiescence, leading to a defect in the maintenance of self-renewal as assessed by serial transplantation....

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