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    Mus musculus
    Michael Rudnicki | 01/12/2012 - 10:54
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of both transcription factors are affected in satellite cell-derived myoblasts (MyoD-/-, p53 -/-).
    Mus musculus
    Michael Rudnicki | 01/12/2012 - 12:00
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    An 11-point time course study comparing V6.5 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days.
    Mus musculus
    T. Michael Underhill | 01/14/2012 - 19:32
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    Comparison of gene expression in differentiating limb bud mesenchymal cells harvested at 0, 1 day and 3 days culture.
    Mus musculus
    Ramesh Shivdasani | 01/24/2012 - 14:21
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430 2.0 Array
    To detect dysregulated gene expression resulting from Cdx2 loss, we isolated RNA from the jejunal epithelium of three 4-8-week old control and 3 Cdx2f/f;Villin- CreERT2 mice 3 days or 4 days after completion of a 5-day course of tamoxifen treatment and followed the manufacturer's instructions to process for hybridization to Mouse Genome 430 2.0 microarrays (Affymetrix).
    Homo sapiens
    James Fleet | 01/26/2012 - 11:55
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U95A v2
    Caco-2 human intestinal cells are widely used to investigate epithelial functions, intestinal gene expression, and transcriptional mechanisms of differentiation. To identify cis-regulatory sequences associated with Caco-2 cell differentiation, we evaluated a distinct epigenetic pattern attributed to active enhancer elements, abundant H3K4Me2 and H3K27Ac marks. To verify CDX2 binding at enhancer regions we used ChIP-seq to identify CDX2 binding sites. Dynamic CDX2 occupancy corresponds with...
    Homo sapiens
    Ramesh Shivdasani | 01/26/2012 - 14:09
    Transcription Factor Binding (ChIP-Seq)
    Illumina Genome Analyzer II
    Caco-2 human intestinal cells are widely used to investigate epithelial functions, intestinal gene expression, and transcriptional mechanisms of differentiation. To identify cis-regulatory sequences associated with Caco-2 cell differentiation, we evaluated a distinct epigenetic pattern attributed to active enhancer elements, abundant H3K4Me2 and H3K27Ac marks. To verify CDX2 binding at enhancer regions we used ChIP-seq to identify CDX2 binding sites. Dynamic CDX2 occupancy corresponds with...
    Homo sapiens
    Ramesh Shivdasani | 01/26/2012 - 14:15
    Histone Modification (ChIP-Seq)
    Illumina Genome Analyzer II
    Caco-2 human intestinal cells are widely used to investigate epithelial functions, intestinal gene expression, and transcriptional mechanisms of differentiation. To identify cis-regulatory sequences associated with Caco-2 cell differentiation, we evaluated a distinct epigenetic pattern attributed to active enhancer elements, abundant H3K4Me2 and H3K27Ac marks. To verify CDX2 binding at enhancer regions we used ChIP-seq to identify CDX2 binding sites. Dynamic CDX2 occupancy corresponds with...
    Mus musculus
    S Prohaska | 02/02/2012 - 14:10
    Transcription Profiling (Microarray)
    Stanford School of Medicine MMAC
    Pairwise comparisons of purified mouse long-term hematopoietic stem cells (LT-HSC), short-term HSC (ST-HSC) and multipotent progenitors (MPP). The hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic subpopulations in adult mouse bone marrow: long-term...
    Mus musculus
    S Prohaska | 02/02/2012 - 22:04
    Transcription Profiling (Microarray)
    Stanford School of Medicine SMD Print_982
    Highly purified Hematopoietic stem cells (HSC) from mouse bone marrow (BM) were compared to HSC after two days of cytoxan/GCSF treatment (Cyclophosphamide/granulocyte colony-stimulating factor) (Day2Mob) and leukemic HSC from mice lacking JunB. See Forsberg et. al. 2010 for details.
    Mus musculus
    Scott Armstrong | 02/04/2012 - 13:28
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome 430A 2.0 Array
    Leukaemias and other cancers possess a rare population of cells capable of the limitless self-renewal necessary for cancer initiation and maintenance. Here we show that leukaemia stem cells (LSC) can maintain the global identity of the progenitor from which they arose while activating a limited stem-cell- or self-renewal-associated programme. We isolated LSC from leukaemias initiated in committed granulocyte macrophage progenitors through introduction of the MLL-AF9 fusion protein encoded by...

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