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Promoting Discovery and Reproducibility in Stem Cell Research

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    Homo sapiens
    John Dick | 12/31/2011 - 20:26
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133A Array
    The effect of low RNA sample amount on amplification fidelity was investigated. RNA concentrations of 7.0 ug/ul, 5.0 ug/ul and 2.0 ug/ul produced from total RNA, 1.0 ug of total RNA, and 40 ng of total RNA, respectively were assayed.
    Homo sapiens
    Michael Rudnicki | 01/03/2012 - 10:44
    Transcription Profiling (Microarray)
    Affymetrix Human Genome U133A Array
    Fetal myoblasts represent an important source of myogenic stem cells. Analysis of undifferentiated myoblasts and comparison to their cognate differentiated progeny will allow the ascertainment of genes that are important for maintaining myogenic stem cells. Fetal myoblasts (undifferentiated, time 0hr) will be compared to myoblasts cells allowed to differentiate by the removal of the SKBM-2 medium + growth factors and feeding with DMEM + 2.5% Horse serum. Time course : 0 hr, 6 hr, 12 hr, 18 hr,...
    Mus musculus
    Michael Rudnicki | 01/03/2012 - 11:00
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    RNA isolates from 10 T1/2 fibroblasts used as baseline control representing gene expression in a terminally differentiated cell type. 10 T1/2 shares a set of genes with stem cells. 10 T1/2 microarray data will be used to subtract these common genes from gene profiles of other stem cell samples. Three best samples to be used for microarray experiment.
    Mus musculus
    Norman Iscove | 01/03/2012 - 11:20
    Transcription Profiling (Microarray)
    Affymetrix Mouse Expression 430A Array
    CD49 Sorted Stem Cells, time 0 after sort, Dulac labeling protocol (NOTE: the dulac protocol amplifies from a small number of cells and the results may be difficult to compare with other more standard microarray analyses.)
    Mus musculus
    John Hassell | 01/03/2012 - 11:38
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    Study of differences in gene expression profiles in the mammary glands tumours of PEA3-null and wild-type PEA3 mice induced by the Her2/Neu transgene.
    Mus musculus
    Janet Rossant | 01/03/2012 - 13:33
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    Comparison of trophoblast stem cells vs. R1 ES cells.
    Mus musculus
    Jacques Galipeau | 01/03/2012 - 14:00
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    These samples, isolated from untransformed marrow stromal cells from wild-type C57BL/6 mice, serve as a control set. The cells (CD31-, CD34 low/-, CD45-) are from a mixed population of 100% bone marrow stromal cells arising after more than two months in culture and 10-15 passages.
    Homo sapiens
    Hans Clevers | 01/03/2012 - 15:47
    Transcription Profiling (Microarray)
    Stanford School of Medicine SHU
    The transactivation of TCF target genes induced by Wnt pathway mutations constitutes the primary transforming event in colorectal cancer (CRC). We show that disruption of beta-catenin/TCF-4 activity in CRC cells induces a rapid G1 arrest and blocks a genetic program that is physiologically active in the proliferative compartment of colon crypts. Coincidently, an intestinal differentiation program is induced. The TCF-4 target gene c-MYC plays a central role in this switch by direct repression of...
    Mus musculus
    Hans Clevers | 01/03/2012 - 16:35
    Transcription Profiling (Microarray)
    Agilent Whole Mouse Genome Microarray 4x44K G4122F
    The small intestinal epithelium is the most rapidly self-renewing tissue of mammals. Proliferative cells are confined to crypts, while differentiated cell types predominantly occupy the villi. We recently demonstrated the existence of a long-lived pool of cycling stem cells defined by Lgr5 expression and intermingled with post-mitotic Paneth cells at crypt bottoms. We have now determined a gene signature for these so called Crypt Base Columnar (CBC) cells. One of the genes within this stem cell...
    Mus musculus
    Lynn Megeney | 01/11/2012 - 09:47
    Transcription Profiling (Microarray)
    Affymetrix Mouse Genome U74A v2
    C2C12 mouse skeletal muscle cells grown in differentiation medium for 5 days.

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