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Homo sapiens
Richard A. Young | 02/25/2015 - 16:53
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer II
Human CD133-positive Umbilical Cord Blood (UCB) cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), PU.1/SPI1 (Santa Cruz SC-352X), IKAROS (Santa Cruz SC-9859X) and MEIS1 (Abcam ab19867).
Mus musculus
Amit Sinha | 02/11/2015 - 10:31
Transcription Factor Binding (ChIP-Seq)
Illumina HiSeq 2000
Leukemic cells from primary and secondary recipients of MLL-AF9-transduced WT and Ezh2-null cells were sorted for GFP and YFP expression and analyzed by Chip-Seq.
Mus musculus
Ramesh Shivdasani | 02/05/2015 - 16:16
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer II
We conditionally inactivated mouse Cdx2, a dominant regulator of intestinal development, and mapped its genome occupancy in adult intestinal villi. Although homeotic transformation, observed in Cdx2-null embryos, was absent in mutant adults, gene expression and cell morphology were vitally compromised. Lethality was accelerated in mice lacking both Cdx2 and its homolog Cdx1, with exaggeration of defects in crypt cell replication and enterocyte differentiation. Cdx2 occupancy correlated with...
Mus musculus
William Pu | 02/05/2015 - 15:27
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer
We performed bio-ChIP-seq using streptavidin beads immunoprecipitation of biotinylated 5 cardiac transcription factors (fbio) and P300 antibody ChIP-seq. We used a dox-inducible dual adenovirus system to express biotinylated Core Cardiac TFs in HL1. One adenovirus expressed the dox-activated reverse tet activator protein (rtTA) and the E. coli biotinylating enzyme BirA from the cardiac specific rat troponin T promoter. The second virus expressed a Core Cardiac TF fused to a C-terminal flag-bio...
Mus musculus
Yi Zhang | 02/05/2015 - 11:46
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer
Polycomb group (PcG) proteins control organism development by regulating the expression of developmental genes. Transcriptional regulation by PcG proteins is achieved at least partly through the PRC2-mediated methylation on lysine 27 of histone H3 (H3K27) and PRC1-mediated ubiquitylation on lysine 119 of histone H2A (uH2A). As an integral component of PRC1, Bmi1 has been demonstrated to be critical for H2A ubiquitylation. Here we report genome wide localization of the Bmi1-dependent uH2A mark...
Homo sapiens
Daniel Tenen | 01/29/2015 - 12:49
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer
RUNX1 siMM and RUNX1 siRE ChIP-Seq samples and a paired-end ChIP-Seq samples from patients with t(8;21) AML are used in this study; there are two paired-end ChIP-Seq runs and control per patient
Homo sapiens
Richard A. Young | 01/28/2015 - 16:32
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer II
Pu.1, MyoD, Oct4, IgG, Smad3, and Smad2/3 ChIP-Seq.
Mus musculus
Richard A. Young | 01/28/2015 - 16:32
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer II
Pu.1, MyoD, Oct4, IgG, Smad3, and Smad2/3 ChIP-Seq.
Homo sapiens
Richard A. Young | 01/26/2015 - 10:31
Transcription Factor Binding (ChIP-Seq)
Illumina Genome Analyzer II
DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against IgG as a background control. ChIP was performed using an antibody against Oct4 in two biological replicates. The first replicate was sequenced twice (technical replicate 1 and technical replicate 2).
Mus musculus
William Pu | 01/22/2015 - 14:55
Transcription Factor Binding (ChIP-Seq)
Illumina HiSeq 2000
Ezh2 and H3K27me3 binding sites in E12.5 heart apex.