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Title: 
Murine expression profiling of activated or control 5-FU bone marrow from MLL-AF4stop knockin animals
Measurement Type: 
Transcription Profiling (Microarray)
Design Type: 
Parallel Group Design
Factors: 
Genetic Characteristics
Summary: 
Mll-AF4stop knock-in mice were treated with 5-FU and 5 days later their bone marrow infected ex vivo with either Cre-GFP to activate the Mll-AF4 fusion construct or with a control MIG-Cre retrovirus. GFP+ cells were sorted 2 days post-infection and cultured for 14 days under lymphoid growth conditions before total RNA was isolated for hybridization to Affymetrix expression microarrays.

Group

Citations: 
H3K79 methylation profiles define murine and human MLL-AF4 leukemias.
Krivtsov AV, Feng Z, Lemieux ME, Faber J, Vempati S, Sinha AU, Xia X, Jesneck J, Bracken AP, Silverman LB, et al.
Cancer Cell. 2008 Nov 4; 14(5):355-68. PMID: 18977325. Abstract
Download: 
Study metadata (ISA-Tab: isa_9768_797596.zip)