H3K79 methylation profiles define murine and human MLL-AF4 leukemias.
Murine expression profiling of activated or control 5-FU bone marrow from MLL-AF4stop knockin animals
Transcription Profiling (Microarray)
Parallel Group Design
Mll-AF4stop knock-in mice were treated with 5-FU and 5 days later their bone marrow infected ex vivo with either Cre-GFP to activate the Mll-AF4 fusion construct or with a control MIG-Cre retrovirus. GFP+ cells were sorted 2 days post-infection and cultured for 14 days under lymphoid growth conditions before total RNA was isolated for hybridization to Affymetrix expression microarrays.
Study metadata (ISA-Tab: isa_9768_797596.zip)