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Molecular Analysis of antigen-specific B cell responses
Measurement Type: 
Transcription Profiling (Microarray)
Design Type: 
Perturbation Design
Time Point, Treatment Type
In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system. Naive B cells were FACS sorted from unimmunized mice, while plasma, germinal center, and memory B cells were sorted from NP-CGG-immunized mice at 1, 2, or 10 weeks after immunization, respectively, using a combination of cell surface markers. RNA was extracted, amplified, and hybridized to Affymetrix microarrays.


Memory T and memory B cells share a transcriptional program of self-renewal with long-term hematopoietic stem cells.
Luckey CJohn, Bhattacharya D, Goldrath AW, Weissman IL, Benoist C, Mathis D
Proc Natl Acad Sci U S A. 2006 Feb 28; 103(9):3304-9. PMID: 16492737. Abstract
Study metadata (ISA-Tab: