Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation.
Heterogeneity of LN resident macrophage
Transcription Profiling (Microarray)
Cell Type/Cell Line
Lymph node (LN) resident macrophages lining the lymphatic sinuses play critical roles in antigen capture and presentation as well as degradation. We used microarray to examine global gene expression profiles to compare subcapsular sinus (SCS) and medullary (med) macrophages to determine the underlying molecular basis of their differential handling of antigens. Primary SCS and med macrophages were isolated and purified from LNs by flourescence-activated cell sorting and RNA extracted, amplified and hybridized for Affymetrix analysis.
Study metadata (ISA-Tab: isa_7534_902962.zip)