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Title: 
Gene expression profiling of normal mouse myeloid cell populations
Measurement Type: 
Transcription Profiling (Microarray)
Design Type: 
Perturbation Design
Factors: 
Cell Surface Markers, Cell Type/Cell Line
Summary: 
Normal myeloid lineage cell populations (C57BL/6 mice, aged 4-10 weeks, male or female) with three distinct immunophenotypes were prospectively isolated and characterized. In preparation for FACS sorting, bone marrow cells were separated into c-kit+ and c-kit- fractions using an AutoMACS device. C-kit+ cells were further fractionated based on Gr1 and Mac1 expression, and absence of lineage antigen expression (B220, TER119, CD3, CD4, CD8 and IL7Rα), by cell sorting. C-kit+ Gr1+ Mac1lo/- and c-kit+ Gr1+ Mac1+ displayed cytologic features of undifferentiated hematopoietic cells or myeloblasts, whereas c-kit- Gr1+ Mac1+ cells were mature neutrophils.
Contact(s): 

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Citations: 
Hierarchical maintenance of MLL myeloid leukemia stem cells employs a transcriptional program shared with embryonic rather than adult stem cells.
Somervaille TCP, Matheny CJ, Spencer GJ, Iwasaki M, Rinn JL, Witten DM, Chang HY, Shurtleff SA, Downing JR, Cleary ML
Cell Stem Cell. 2009 Feb 6; 4(2):129-40. PMID: 19200802. Abstract
Download: 
Study metadata (ISA-Tab: isa_7518_739408.zip)