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Title: 
Differentially regulated genes in normal LT-HSC vs ST-HSC
Measurement Type: 
Transcription Profiling (Microarray)
Design Type: 
Perturbation Design
Factors: 
Cell Type/Cell Line
Summary: 
Self-renewal is a defining characteristic of stem cells, however the molecular pathways underlying its regulation are poorly understood. Here we demonstrate that conditional inactivation of the Pbx1 proto-oncogene in the hematopoietic compartment results in a progressive loss of long-term hematopoietic stem cells (LT-HSCs) that is associated with concomitant reduction in their quiescence, leading to a defect in the maintenance of self-renewal as assessed by serial transplantation. Transcriptional profiling revealed that multiple stem cell maintenance factors are perturbed in Pbx1-deficient LT-HSCs, which prematurely express a large subset of genes, including cell cycle regulators, normally expressed in non-self-renewing multipotent progenitors. LT-HSC (Lin-cKit+Sca1+CD34-CD135-) and ST-HSC (Lin-cKit+Sca1+CD34+CD135-) cells were prospectively sorted from the BM of MxCre-.Pbx1f/f control mice harvested 4 weeks after the last injection of poly(I:C).
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Citations: 
Pbx1 regulates self-renewal of long-term hematopoietic stem cells by maintaining their quiescence.
Ficara F, Murphy MJ, Lin M, Cleary ML
Cell Stem Cell. 2008 May 8; 2(5):484-96. PMID: 18462698. Abstract
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Study metadata (ISA-Tab: isa_7283_610323.zip)