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Title: 
Transcription factor ChIP-seq in expanded human hematopoietic stem and progenitor cells
Measurement Type: 
Transcription Factor Binding (ChIP-Seq)
Summary: 
Human CD133-positive Umbilical Cord Blood (UCB) cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), PU.1/SPI1 (Santa Cruz SC-352X), IKAROS (Santa Cruz SC-9859X) and MEIS1 (Abcam ab19867).
Contact(s): 
Curator(s): 
mmerrill

Group

Citations: 
Densely interconnected transcriptional circuits control cell states in human hematopoiesis.
Novershtern N, Subramanian A, Lawton LN, Mak RH, W Haining N, McConkey ME, Habib N, Yosef N, Chang CY, Shay T, et al.
Cell. 2011 Jan 21; 144(2):296-309. PMID: 21241896. Abstract
Download: 
Study metadata (ISA-Tab: isa_16554_582916.zip)