Densely interconnected transcriptional circuits control cell states in human hematopoiesis.
Transcription factor ChIP-seq in expanded human hematopoietic stem and progenitor cells
Transcription Factor Binding (ChIP-Seq)
Human CD133-positive Umbilical Cord Blood (UCB) cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), PU.1/SPI1 (Santa Cruz SC-352X), IKAROS (Santa Cruz SC-9859X) and MEIS1 (Abcam ab19867).
Study metadata (ISA-Tab: isa_16554_582916.zip)