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Title: 
Analysis of H3K79 methylation during reprogramming
Measurement Type: 
Transcription Factor Binding (ChIP-Seq)
Factors: 
Treatment Type, Immunoprecipitation Antibody
Summary: 
Inhibition of the histone 3 lysine 79 (H3K79) methyltransferase increases reprogramming efficiency and genome-wide analysis of H3K79me2 distribution revealed that fibroblast-specific genes associated with the epithelial to mesenchymal transition lose H3K79me2 in the initial phases of reprogramming. Dot1L inhibition facilitates the loss of this mark from genes that are fated to be repressed in the pluripotent state. Chromatin immunoprecipitation followed by Solexa sequencing for H3K27me3 and H3K79me2 in Fibroblasts, Embryonic stem cells, and fibroblast undergoing reprogramming.
Curator(s): 
mmerrill

Group

Citations: 
Chromatin-modifying enzymes as modulators of reprogramming.
Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, B Marcarci O, Unternaehrer J, Gupta PB, et al.
Nature. 2012 Mar 29; 483(7391):598-602. PMID: 22388813. Abstract
Download: 
Study metadata (ISA-Tab: isa_15849_655437.zip)