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Title: 
ChIP-Seq of TAL1 and its regulatory partners in T-ALL cells
Measurement Type: 
Transcription Factor Binding (ChIP-Seq)
Factors: 
Immunoprecipitation Antibody
Summary: 
Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total TAL1 (Santa Cruz SC-12984), TCF12/HEB (Santa Cruz SC-357),TCF3/E2A (Santa Cruz SC-349X), LMO1 (Santa Cruz SC-10494), LMO2 (R&D AF2726),GATA3 (Santa Cruz SC-22206) and RUNX1 (Santa Cruz SC-8563). This represents the ChIP-seq portion of this dataset.
Contact(s): 
Curator(s): 
mmerrill

Group

Citations: 
Core transcriptional regulatory circuit controlled by the TAL1 complex in human T cell acute lymphoblastic leukemia.
Sanda T, Lawton LN, M Barrasa I, Fan ZPeng, Kohlhammer H, Gutierrez A, Ma W, Tatarek J, Ahn Y, Kelliher MA, et al.
Cancer Cell. 2012 Aug 14; 22(2):209-21. PMID: 22897851. Abstract
Download: 
Study metadata (ISA-Tab: isa_15819_631807.zip)